Frank Lampard is a major doubt for Euro 2012 after suffering a thigh injury in training on Wednesday morning.
The Chelsea midfielder will undergo a scan on Thursday, having only just joined the squad after being given a week off to rest after the Champions League final.
Lampard had only just joined up with the England squad after an extended break following Chelsea's Champions League final victory.
He will be assessed by Roy Hodgson's medical team before any decision is taken about his participation in the finals in Poland and Ukraine.
England's first group match against France is just 12 days away and Hodgson can ill-afford any more injury problems in midfield.
Manchester City's Gareth Barry was ruled out of the Euros with a tear of his lower abdomen.
Hodgson has already gambled on the fitness of Manchester United striker Danny Welbeck, who hasn't played since the end of April because of an ankle injury.
Liverpool defender Glen Johnson's fitness has also been a doubt after suffering from an infected toe.
Hodgson has one more friendly, against Belgium at Wembley on Saturday, before his 23-man squad fly to their Polish base next week.