Campaigners camped outside St Paul's Cathedral are planning a "peaceful resistance", having been refused the right to challenge their eviction.
Three judges headed at the Court of Appeal dismissed applications to appeal against a ruling in the High Court last month.
The protesters have been told to leave immediately.
An 'emergency meeting' of the camp's General Assembly has been called this evening to discuss what to do next.
Legal representatives were walking around the camp today, handing out florescent pink cards with information about options and laws with regards to an eviction.
Instructions include: if resisting peacefully - carry a 'cheap phone' and no ID.
One campaigner called 'Catherine' told Sky News "It is disappointing that this is going. This has seemed like a second home to me. I think there will be a lot of peaceful departure and peaceful resistance. I hope there's no violence."
The City of London Corporation has said it will now enforce the orders made by the High Court for the removal of the tents and equipment.
But it will give no indication of when that might happen for "operational reasons".
Speaking to Sky News, local MP Mark Field said: "I'm very pleased with the outcome.
"Given that all sides have exhaustedly gone through the legal avenues, it is incumbent on the protesters to move on peacefully from the site.
"With the Diamond Jubilee and the Olympics this year we need to get the site outside the iconic St Paul's cleaned up."
St Pauls Cathedral released a statement saying: "We very much hope that today's decision by the Court of Appeal will now lead to a peaceful dispersal of the camp outside St Paul's.
"For the past five months we have sought to focus on the important ethical issues raised by the Occupy London movement, and we remain committed to engage with these issues."
Protesters have been camped at the location since October 15 as part of worldwide action inspired by the Occupy Wall Street movement in the US.
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