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The 23-year-old singer has re-listed the track on iTunes, with all proceeds going to the We Love Manchester Emergency Fund.
A message posted alongside the release of her new single read: “Some of the world’s biggest pop stars – including Justin Bieber, Coldplay, Katy Perry, Miley Cyrus, Pharrell Williams, Take That, Niall Horan, Little Mix, Robbie Williams and Black Eyed Peas – join forces for Ariana Grande’s One Love Manchester concert to support those affected by the tragic events of May 22.
“Stand united with Manchester: download Ariana’s ‘One Last Time’ and watch the concert live on Sunday on Apple Music.
“All proceeds from each sale of the track featured on the page will be donated to the We Love Manchester Emergency Fund in coordination with the British Red Cross.”
Countless victims suffered serious injury with 22 others tragically losing their lives.
Devastated Ariana jetted home to Florida shortly after the attack took place in order to be with her family, however, she then organised a benefit concert, One Love Manchester, to be held at Emirates Old Trafford cricket ground later tonight.
All proceeds from the concert – which will include performances from A-list stars such as Katy Perry and Justin Bieber – will go to those who have been affected by the terror attack, with Ariana also making the time to visit victims in hospital at the weekend.
Flying into London on Friday morning, Ariana drove straight to Manchester where she visited her hospitalised fans, giving them sunflowers and gifts, including a Harrods teddy bear.
The star stayed to chat with the victims as well as members of hospital staff who worked tirelessly to save them.
You can watch One Love Manchester live on BBC One tonight from 6.55pm.