The tie is finely poised at 1-1 after goals from Dries Mertens and Antoine Griezmann in the initial pre-lockdown fixture played all the way back in February.
Head coach Quique Setien's Barca future could depend on European success after his side were beaten to the LaLiga title by fierce rivals Real Madrid this term.
With kick-off at 8pm BST, follow all the action with Ben Hayward!
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Date, time and venue
Barcelona vs Napoli is scheduled for a 8pm BST kick-off on Saturday, August 8, 2020.
The match will be played behind closed doors at Camp Nou with no fans due to be present due to government guidelines regarding coronavirus.
How to watch
TV channel: The match will be televised live on BT Sport 2, with coverage starting from 7:30pm.
Live stream: BT Sport subscribers will be able to stream the match online via the video player and the BT Sport app.
Barcelona XI: ter Stegen; Semedo, Pique, Lenglet, Jordi Alba; Rakitic, Roberto, De Jong; Messi, Suarez, Griezmann
Prediction: Barcelona 1-0 Napoli (2-1 on aggregate)
Barca have thrown away numerous second-leg ties in recent years, and Setien's job depends on going all the way this year.
Head to head (h2h) history and results
The two sides have only met once previously in Uefa competitions - in the first leg of this last-16 tie in Naples.
Antoine Griezmann sealed a draw with an away goal after Dries Mertens opened the scoring for Napoli.