After beating the Czech Republic to the runners-up spot behind England in Group D, 2018 World Cup finalists Croatia have been dealt a huge blow with the news that Ivan Perisic has tested positive for Covid-19.
The influential, 104-cap Inter Milan forward - who played all but nine minutes of the group stage campaign, scoring against both the Czechs and Scotland - has entered a 10-day period of self-isolation and would also stand to miss the quarter-finals and semis, should Zlatko Dalic’s side advance that far at a second successive major tournament.
AC Milan winger Ante Rebic replaces Perisic, returning to the attack alongside Nikola Vlasic and Bruno Petkovic.
Duje Caleta-Car also replaces Dejan Lovren in defence, while Atletico Madrid’s Sime Vrsaljko has not regained his right-back spot from Josip Juranovic.
As for Spain, an uninspiring campaign that began with successive low-key draws ignited with a 5-0 drubbing of Slovakia in Seville as they eventually claimed second place in Group E behind Sweden.
Here is how the two sides will shape up in Copenhagen...
Croatia vs Spain starting lineups
Croatia XI: Livakovic; Juranovic, Vida, Caleta-Car, Gvardiol; Modric, Brozovic, Kovacic; Vlasic; Rebic, Petkovic
Spain XI: Simon; Azpilicueta, Eric Garcia, Laporte, Gaya; Koke, Busquets, Pedri; Sarabia, Morata, Ferran
Goalkeepers: Lovre Kalinic, Dominik Livakovic, Simon Sluga
Defenders: Domagoj Vida, Dejan Lovren, Sime Vrsaljko, Borna Barisic, Duje Caleta-Car, Josip Juranovic, Domagoj Bradaric, Mile Skoric, Josko Gvardiol
Midfielders: Luka Modric, Mateo Kovacic, Marcelo Brozovic, Milan Badelj, Mario Pasalic, Nikola Vlasic, Luka Ivanusec
Forwards: Ivan Perisic, Andrej Kramaric, Ante Rebic, Josip Brekalo, Bruno Petkovic, Mislav Orsic, Ante Budimir
Goalkeepers: David de Gea, Robert Sanchez, Unai Simon
Defenders: Aymeric Laporte, Jose Gaya, Jordi Alba, Pau Torres, Eric Garcia, Diego Llorente, Cesar Azpilicueta
Midfielders: Marcos Llorente, Sergio Busquets, Rodri, Pedri, Thiago, Koke, Fabian
Forwards: Dani Olmo, Mikel Oyarzabal, Gerard Moreno, Alvaro Morata, Ferran Torres, Adama Traore, Pablo Sarabia
Duje Caleta-Car, Marcelo Brozovic and Mateo Kovacic (Croatia) and Pau Torres, Sergio Busquets, Rodri and Jordi Alba (Spain) will miss the next match if they are booked.