Wilder, who shocked the boxing world this week after commenting he wants "a body on his record", puts his belt on the line for the ninth time.
Breazeale, who challenged for Anthony Joshua’s IBF crown in 2016 when he was knocked out in the seventh round, became the mandatory challenger for Wilder’s title with a KO win over Carlos Negron in Brooklyn late last year.
Follow all the action with Standard Sport's LIVE blog!
Date, UK start time and venue
Wilder vs Breazeale takes place tonight at the Barclays Center in New York.
It is another early start for UK fight fans with ringwalks not expected until no earlier than 4am BST in the early hours of Sunday morning.
TV channel and live stream
The fight will be shown live on Sky Sports' Action and Main Event channels, and is available via Now TV with a day pass on offer for £8.99.
Sky Sports subscribers can stream the match online via SkyGo and the SkyGo app.
Of the heavyweight's division's Big Three, Wilder arguably faces the toughest challenge in the coming weeks in Breazeale compared to Andy Ruiz Jnr and Thomas Schwarz, who come against Joshua and Fury respectively.
Having said that, Wilder should be confident of registering victory no. 41. A win via a late stoppage for the defending champion.
• Gary Russell Jr vs Kiko Martinez (WBC world featherweight title)
• Juan Heraldez vs Argenis Mendez (lightweight)
• Robert Alfonso vs Iago Kiladze (heavyweight)
• Gary Antonio Russell vs Saul Eduardo Hernandez (bantamweight)
• Gary Antuanne Russell vs Marcos Mojica (super lightweight)
• Kenny Robles vs Roy McGill (super lightweight)
• Dylan Price vs Manuel Salvador Manzo (super flyweight)