The Earl and Countess of Wessex have paid tribute to the Queen, saying there's an "unimaginable void in all our lives".
They added a personal touch, referring to the Queen as their "beloved mama".
In a statement, Edward, the Queen's youngest son, and his wife Sophie said they have been "overwhelmed by the tide of emotion" after the monarch's death, adding: "Thank you for your support, you have no idea how much it means".
They continued: "The Queen's passing has left an unimaginable void in all our lives.
"Sophie and I have taken huge pleasure in seeing our James and Louise enjoying the places and activities that their grandparents loved so much.
"Given that my mama let us spend so much time with her, I think she also rather enjoyed watching those passions blossom.
"Those times together, those happy memories, have now become massively precious to each and every one of us."
The couple are the latest members of the Royal Family to publicly speak about the Queen following her death.
The Prince of Wales, now heir to the throne, said: "So much will be said in the days ahead about the meaning of her historic reign.
"I, however, have lost a grandmother. And while I will grieve her loss, I also feel incredibly grateful. I have had the benefit of the Queen's wisdom and reassurance into my fifth decade."
Meanwhile, thousands of people will continue to queue to see the Queen lying in state at Westminster Hall.
The queue to see the coffin was shut for over seven hours on Friday morning after reaching capacity - stretching 4.9 miles with a wait time of 14 hours, the Department for Digital, Media, Culture and Sport said. The queue reopened just after 5pm, with a new wait time of over 24 hours.