The Three Lions currently lead Group F with three wins and a draw from their opening four matches, but failure to beat Edgaras Jankauskas's Lithuania could cost them top spot.
The teams and London mayor Sadiq Khan will also pay tribute to the victims of Wednesday afternoon's terror attacks in London, with the FA confirming that the "bravery" of those affected will be honoured at Wembley.
England: Hart, Walker, Keane, Stones, Bertrand, Dier, Oxlade-Chamberlain, Lallana, Alli, Sterling, Defoe.
Subs: Forster, Heaton, Clyne, Shaw, Gibson, Livermore, Ward-Prowse, Barkley, Vardy, Rashford, Lingard, Redmond.
Lithuania: Setkus, Vaitkunas, Kijanskas, Linas Klimavicius, Slavickas, Kuklys, Zulpa, Novikovas, Slivka, Cernych, Valskis.
Subs: Zubas, Gertmonas, Mikuckis, Grigaravicius, Verbickas, Matulevicius, Paulius, Arunas Klimavicius, Eliosius , Borovskij, Luksa, Baravykas.
Referee: Ruddy Buquet (France)
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WHAT THEY SAID
Gareth Southgate: "We know how important the national football team is for the feeling of the nation, and it's a great opportunity for everybody to pay their respects at that moment, and to show we go on," said Southgate.
"Not just London, but the nation. That's our identity, historically. I thought the tribute before the France game was very poignant. This will be the same."
Edgaras Jankauskas: "First of all it's a privilege to play at such a legendary stadium and probably one of the best stadiums in the world.
"There are always chances and chances depend on us, (there are) many examples where not necessarily the favourites win the game. We are a humble team but we have our game plan and we'll stick to that.