Spurs cut the Blues title lead to four points with a 4-0 win over Bournemouth at White Hart Lane.
Mousa Dembele converted Christian Eriksen's corner inside 20 minutes before Heung-min Son latched on to Kane's through ball to net his 18th goal of the campaign.
And Kane, who came off the bench to make his injury return in the thumping win against Watford last weekend, rolled in a third to cap his first start since a 27-day lay-off.
EPL | The Premier League table after 32 matches
Vincent Janssen completed the rout in injury time with his first Premier League goal from open play to seal a second straight 4-0 win at White Hart Lane.
Leaders Chelsea can again extend their title lead to seven points when they face Manchester United at Old Trafford on Sunday.
Elsewhere on Saturday, Crystal Palace host champions Leicester, and West Ham go to Sunderland.
Arsenal will then seek to avoid another Monday-night drubbing as they travel to Middlesbrough with their top-four hopes hanging by a thread.