Transport for London said crowds would be held in Wembley Way and only allowed into Wembley Park station at a slower than normal pace to prevent Metropolitan and Jubilee line trains becoming overcrowded.
Between 40,000-45,000 fans are expected to attend the Round of 16 match, which kicks off at 5pm. Fans were also told they would be denied access to the station if they failed to wear a face mask.
Andy Lord, managing director of London Underground, said: “We urge fans to be patient as it may take longer than usual to access stations after the games. Customers must please wear a face covering over their nose and mouth unless they are exempt.”
Wembley is also due to host both semi-finals and the final next week, with about 67,000 fans expected to be able to attend.
The semi-finals are being held on Tuesday and Wednesday next week, July 6 and 7. The final is on Sunday July 11.
All three matches kick-off at 8pm and TfL chiefs are fearful of fans missing the last Tube at around midnight if the games go to extra time and penalties.
Mr Lord said: “Fans travelling to the games should ensure that they check their journeys before they travel and leave enough time to get to Wembley so they don’t miss kick off, and should make plans in case the games go to extra time and penalties.
“A huge amount of work has been put in to make sure that journeys can be completed safely and our staff will be on hand in stations to help customers.”
Londoners not attending the matches are advised to avoid the area if possible. The Bakerloo line and London Overground, which also serve the stadium, are also expected to be busier than normal.
Face coverings will be handed out by TfL staff at Wembley Park and Wembley Central stations after games for customers who do not have one.
TfL said that the failure to wear a mask meant that passengers may be denied travel or be fined a minimum of £200.