The Larne to Cairnryan route has been interrupted in recent weeks after the company replaced nearly 800 seafarers with cheaper agency staff.
The sackings sparked anger among unions and political representatives.
#POCairnryan / #POLarne: Our services have now resumed sailing. Please see the current schedule for departures from:
Larne: 08:00, 12:00, 16:00, 20:00, 23:59.
Cairnryan: 12:00, 16:00, 20:00, 23:59.
We look forward to welcoming you on board.
— P&O Ferries Updates (@POferriesupdate) April 24, 2022
The European Highlander ferry, which is one of two ferries which operate on the Irish Sea route, was subjected to an inspection by the Maritime and Coastguard Agency.
On Sunday morning P&O informed customers via Twitter that the services from Larne to Cairnryan have resumed sailing.
Five departures from Larne and four departures from Cairnryan were expected to take place on Sunday.
The European Causeway and the European Highlander ferries were operating on the route on Sunday afternoon according to the Marine Traffic app.