Sophie Tinger, 10, from Hertfordshire, was inspired to sing the song after it was referenced in the Queen's national address.
She sent the video to her cousins who work for the NHS and the video was shared online by one of them, Andrew Tenzer.
Sophie begins the video by saying: "Hi everybody, I hate this isolation and quarantine thing so I’m going to cheer you up by doing a nice song called We’ll Meet Again by Vera Lynn.
"Anyone who’s watching from the NHS I just want to say I really appreciate your work. Thank you so much for looking after us."
On Sunday, more than 23.5 million viewers watched the Queen’s televised coronavirus address to the nation.
The Queen referenced Vera Lynn's song in her speech, saying: “We will succeed – and that success will belong to every one of us.
“We should take comfort that while we may have more still to endure, better days will return: we will be with our friends again; we will be with our families again; we will meet again."
The song has since climbed the charts, ranking number 22 on the UK charts.
Sophie originally learned the song to perform to elderly people at care homes with a friend, but when the Covid-19 pandemic prevented her from carrying out the visits she decided to perform the song for a different audience.
She told Mirror Online: "This song means a lot to me because I would like to meet my friends and family again but I don't know where and when and [it] gives people joy and hope like it did in the war.
"I think they are so brave and bold to go to work where they could catch coronavirus.
"While we are just staying at home they are working day and night and I am so grateful to them."