The 76-year-old Rocket Man star singer is set to close the music festival, headlining the famous Pyramid Stage on Sunday, 25 June.
It is due to be the final UK performance of John's Farewell Yellow Brick Road retirement tour, which began in 2018 and will finally end in Sweden on 8 July.
Glastonbury Festival has become known for signing up older, enduring male stars to headline such as Sir Paul McCartney, The Rolling Stones, The Who, Neil Young, Bruce Springsteen and Stevie Wonder.
So how many women have headlined the Pyramid Stage in the last 20 years?
We count them on one hand...
Billie Eilish - 2022
Not only was she flying the flag for female kind, Billie Eilish made history as the youngest ever headline act at Glastonbury Festival when she performed on opening night in 2022.
The 21-year-old Your Power singer played the Pyramid Stage on the Friday night as the festival made its great comeback after the coronavirus pandemic forced its cancellation for two years running.
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Eilish is only the fifth female headliner out of 45 acts in the past 20 years. And one of those was not the official headline act the year they performed, but were moved up the billing due to unforeseen circumstances.
Adele - 2016
Adele was the big booking of Glastonbury 2016 - headlining the Pyramid Stage on Saturday night that year.
The Hometown Glory singer was very emotional about performing at the historic music venue.
Radio 1 DJ Jo Whiley revealed: "She was really scared, really, really scared. We were doing the interview and at one point she had to stop as she was in tears.
"She was saying how much Glastonbury meant to her, and she'd been going since she was a child."
Florence and The Machine - 2015
The Foo Fighters had been due to headline the mainstage on Friday night at Glastonbury 2015. But three weeks before the festival it was revealed that frontman Dave Grohl had fallen off the stage at a gig in Sweden and broken his leg - forcing the band to cancel a number of appearances.
So Florence and the Machine were announced as the replacement headliner 10 days later and Florence Welch became the seventh female act to headline the Pyramid stage 45 years after the festival began.
Florence and the Machine performed a cover of the Foo Fighters' Times Like These as part of their set and dedicated it to Grohl.
Beyonce - 2011
Three years after her husband Jay-Z had headlined Glastonbury, the festival managed to persuade the Queen Bey to grace the Pyramid Stage on Friday night, helping girls see they can Run The World and headline music festivals.
Beyonce was already eight weeks pregnant with daughter Blue Ivy when she performed at Glasto.
Her pal Gwyneth Paltrow later revealed the Crazy In Love singer was suffering from terrible morning sickness.
Paltrow said: "She was absolutely incredible, especially as she was barfing in between."
Kylie Minogue - replaced by Basement Jaxx in 2005
The Spinning Around star has appeared at Glastonbury several times since, but never in the headline slot on the Pyramid Stage.
That same year Meg White headlined on the opening night with The White Stripes, drumming up some female representation.
Taylor Swift - cancelled in 2020
Taylor Swift was booked to headline the Pyramid Stage on Saturday night in 2020 but the festival was cancelled due to the coronavirus pandemic. Sir Paul McCartney and Kendrick Lamar rescheduled their performances for the 2022 festival, but Swift decided to Shake It Off.
In the 53 years since the first Glastonbury, over 38 festivals, there have only been a handful of other women to secure the headline slot on the Pyramid Stage.
These include Sinead O'Connor, Shakespears Sister, Suzanne Vega and Skunk Anansie.
That's a total of nine acts.
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