The cast of Glee will release their version of Band Aid's Do They Know It's Christmas? after it is performed on tonight's show - donating all monies to the charity.
The Sky1 show is expected to raise millions of pounds for Band Aid which was set up by Sir Bob Geldof to raise money for children living in poverty in Africa.
Written by Geldof and Midge Ure, Do They Know It's Christmas? was first recorded in 1984.
The pair recruited some of the biggest music stars of the time, including Sting, Bono, George Michael , and Duran Duran's Simon LeBon.
It went straight to number one after becoming the fastest-selling single in the UK.
Geldof says the "power" of Glee will help expose the song and its meaning to a "giant worldwide audience of young people".
He is hopeful the show will make people think about one of the "great issues of the 21st century" which sees over half the people in the world "living in intolerable poverty."
Bono said his involvement with the track shaped his future charity work.
"This song did turn my life upside...can a song change the world? Well it certainly changed my world...Everything I do now started with that little song, Do They Know It's Christmas?"
Sting remarked: "It's still something we need to get out now and again for a new generation."
The show has pledged to donate 100 percent of its net monies from the sales of the cover to Band Aid.
It will also feature on Glee's Christmas album.