Home and Away star Jessica Falkholt has died from injuries sustained in a Boxing Day car crash.
The 29-year-old, who played Hope Morrison in the soap, died on Wednesday morning, St George Hospital in Sydney confirmed.
The crash also claimed the lives of her parents Lars and Vivian and her sister Annabelle.
Falkholt had been in a medically-induced coma since the collision in New South Wales, with family members keeping vigil at her bedside.
A spokeswoman said: "Jessica passed away from serious injuries sustained in a motor vehicle accident on 26 December, 2017.
"The family has asked for privacy during this very difficult time."
Channel Seven, the Australian broadcaster that produces the show, said the cast are "heartbroken" to learn of her death.
"Jessica's shining talent was recognised and respected by all her cast mates and crew and her beautiful friendship treasured," a spokesman added.
"We send our sincere condolences to her extended family and friends during this difficult time."
Falkholt's parents Lars and Vivian were also killed in a head-on crash - while her sister Annabelle was critically injured and died three days later in hospital.
The soap's stars paid tribute to her, with Brody Morgan actor Jackson Heywood saying her "beautiful soul will be so deeply missed".
Pia Miller, who played Katrina Chapman, said: "Farewell wonderful Jess."
Falkholt's agent Lisa Mann said the news was "heart breaking", adding: "Beautiful Jess was loved and treasured by her family, friends and colleagues and our sympathies are with everyone today."
Emergency services were called to Princes Highway at Monday on on 10.45am on December 26 with three people pronounced dead at the scene.
The driver of the other car, Craig Whitall, also died.