The Spurs striker is sidelined with an ankle ligament injury, and it not expected to return before the end of April, to rule him out of the World Cup qualifier against Lithuania.
Gareth Southgate and assistant Steve Holland took charge of the session, which featured three of Kane's Tottenham team-mates in Kyle Walker, Eric Dier and Dele Alli.
Kane is joined on the sidelines, for both Tottenham and England, by Danny Rose as the Three Lions prepare to face Edgaras Jankauskas's team at Wembley on Sunday.
Southgate was quizzed on Kane's importance to the squad when addressing reporters after the session, replying with: "It's great that we have a player like that still to come in."
England sit top of Group F with three wins and a draw from games against Scotland, Malta, Slovakia and Slovenia, with two points separating themselves from the second-placed Slovaks.
Southgate geared up for Sunday's game with, in his first game in charge since being appointed manager on a permanent basis, a 1-0 loss to Germany in Dortmund.
Lukas Podolski, on his 130th and last international outing, scored the only goal with a thumping 30-yard effort in the second half.