One of the two controversial Netflix movies opening at the French festival has been booed just five minutes into a screening.
Bong Joon-ho's sci-fi drama Okja had to be stopped, after the screening curtains malfunctioned, hiding part of the screen.
But the whistling, booing and jeering started before the movie.
"Ha, this is really not made for the cinema," one spectator shouted.
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"Okja starts, huge boos at Netflix logo. Then film plays in wrong aspect ratio and Grand Lumiere almost rioted. Movie stopped," wrote Indiewire's David Ehrlich on Twitter.
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In a statement posted on its official Twitter account, the festival said "a technical incident" had "disrupted the beginning of the screening".
"This incident was entirely the responsibility of the Festival's technical service which offers its apologies to the director, his teams, the producers and the audience at the showing."
On Wednesday, the same happened with Todd Haynes' Wonderstruck, when the Amazon Studios logo appeared on screen.
The backlash against the two streaming giants comes as Netflix refused to premiere its two Palme d'Or contenders in French cinemas.
Both Okja and Noah Baumbach's The Meyerowitz Stories are running for Cannes' top prize, but the festival has warned it is the last year streaming-first movies will be able to compete.
Netflix says it reserves the rights to premiere its own movies on its platform.
According to French law, a movie which opens in a French cinema has to wait three years until it is available for streaming.
The row took an unexpected turn on Wednesday , when jury president Pedro Almodovar said he would not give the two Netflix movies a chance to compete for the Palme.
He said he could not imagine "the Palme d'Or nor any other prize being given to a film, and then not being able to see that film on a large screen".
But his fellow jury member Hollywood star Will Smith took a strongly opposing view, and said he was "looking for the opportunity to slam my hand on the table and disagree with Pedro".
"I'm looking forward to a good jury scandal."
Okja tells the story of a young girl who befriends a large, man-made beast meant for the meat industry.
South Korean director Bong Joon-ho has been hailed by Quentin Tarantino as our generation's Steven Spielberg.