Disturbing footage shared online shows the men spitting at the bus and punching windows while shouting slurs.
London Mayor Sadiq Khan condemned the attack, saying antisemitism had “no place whatsoever in society”.
He added: “I utterly condemn these disgusting acts. No one should have to experience this.
“If you have any information about this incident, I urge you to report it.”
Officers stopped the bus in Grosvenor Place to speak with passengers about the incident and to check on their welfare. There were no reports of any injuries.
Detective Kevin Eade, from the local policing team in Westminster, said: “Our investigation into this appalling incident continues and we are now in a position to release three clearer of images of the men would like to speak to.
“Despite extensive enquiries over the past week, we are yet to make any arrests, however I am confident that somebody will recognise the people in these images, and I would urge anyone who does to contact us immediately."
Anyone with information is asked to call police on 101 or tweet @MetCC quoting 6184/29Nov. Alternatively, call the Charing Cross Hate Crime Unit direct on 07900 608 252 [during office hours only] or email AWMailbox-.HateCrimeUnit@met.police.uk
To remain anonymous contact the independent charity Crimestoppers on 0800 555 111.