Bhupinder Singh Gill has made Premier League history by becoming the first Sikh-Punjabi to serve as an assistant referee.
The official ran the line at Southampton's game against Nottingham Forest on Wednesday evening.
He joined fellow assistant referee Marc Perry and match referee Thomas Bramall for the game.
The PE teacher is the son of Jarnail Singh, the first turbaned referee in English league football history.
Jarnail Singh took charge of more than 150 English Football League (EFL) games between 2004 and 2010, but none in the Premier League, Sky Sports said.
His brother is Sunny Singh Gill, who earlier this season became the first British South Asian to referee an EFL game since the pair's father.
Mr Singh told the Premier League's website: "Hopefully, this is another moment to help inspire the next generation to sign up to a refereeing course and get into officiating.
"My dream has always been to reach the top of the game, be a role model for future officials and encourage more people from diverse backgrounds into officiating, especially from a South Asian background just like me."
Speaking before the game, he said: "This has to be the proudest and most exciting moment in my refereeing journey so far, but I'm not getting carried away as it is just another step in the direction to where I want to get to.
"My family are also really proud and excited for me. I wouldn't be in this situation if it wasn't for my dad, who has supported me throughout my journey and been a role model for me."
Jarnail Singh, who was in the crowd at St Mary's, told Sky Sports News: "For the Sikh community and all South Asians who dream of a career in the game, seeing Bhupinder officiate in the Premier League is absolutely massive.
"As a father, you can imagine I am very proud to see Bhupinder in the Premier League. It's every father's dream to see their children succeed, and do better than them, and achieve more - whether that is in terms of family life, education or sport.
"Hopefully, Bhupinder's achievement highlights what you can do with hard work, endeavour and passion for the game."
Forest won the game 1-0.