Goals by Marcus Rashford and Ander Herrera sank the league leaders and, with six games left for the top two, there is little room for any more slip-ups by Antonio Conte's side who a few weeks ago looked like they were running away with the title.
Chelsea remain favourites to win the league, though Conte will be concerned about the manner of the defeat at Old Trafford where they were rarely able to turn possession into chances as Spurs - having won seven on the trot - gain momentum.
The west London side are top with 75 points ahead of Spurs on 71, also from 32 games, Liverpool with 66 from 33, Manchester City on 64 from 32 and United with 60 from 31.
BETTING ODDS (via 888Sport)
Premier League title race
- Chelsea: 9/50
- Spurs: 9/2
- Liverpool: 200/1
- Man City: 200/1
- Man Utd: 1000/1
- Arsenal: 2500/1
Click here to place a bet. Odds correct as of April 16 at 6pm.