Five patients have died from the infection after eating pre-packaged sandwiches at hospitals across the country.
The latest two deaths took place at University Hospitals of Derby and Burton NHS Foundation Trust and University Hospitals of Leicester NHS Trust.
Public Health England also revealed that the Good Food Chain, who supplied the contaminated sandwiches, had sent affected products to a further 43 NHS Trusts, as well as one independent provider.
The first two deaths were at Manchester University NHS Foundation Trust, while a third happened at Aintree Univesrity Hospital NHS Foundation Trust.
Other cases, though not fatal, have been identified at Western Sussex Hospitals NHS Foundation Trust (two cases), Frimley Health NHS Foundation Trust and East Kent Hospitals University NHS Foundation Trust.
The business was supplied with meat produced by North Country Cooked Meats, which has since tested positive for the outbreak strain of listeria and also stopped production.
Listeria infection is rare and usually causes a mild illness in healthy people.
However, it can have more serious consequences among those with pre-existing medical conditions, pregnant women and those with a weak immune system.
The first case showed symptoms on April 25 and sandwiches and salads were withdrawn on May 25, as soon as a link with the cases was suspected.
It is understood that some of the products were sold at hospitals while others were given to patients.
Health Secretary Matt Hancock ordered a review of NHS food after the outbreak was made public.
He said he was "incredibly concerned" and that "staff, patients and families deserve so much better."
Mr Hancock added: "I have been incredibly concerned by this issue and strongly believe that we need a radical new approach to the food that is served in our NHS.
"Staff, patients and families deserve so much better - our NHS should be at the forefront of supporting people to make healthy choices.
"I have instructed the NHS to conduct a root and branch review of hospital food."