It had been rumoured the outspoken sibling of the Duchess of Sussex would be taking part in the Channel 5 reality show.
Since the show’s launch, last Thursday, speculation has been growing over whether Ms Markle would replace Stormy Daniels who sensationally quit moments before the series kicked off.
On Saturday, she took to Twitter to set the record straight.
Ms Markle said: “This just in: Not going on #CBB considering I other opportunity. All up in the air LOL”
One person accused Ms Markle of lying over appearing on the show on Twitter.
She hit back, saying: “I was not the one who started that one” and added: “There would not be enough room in that house for my mouth.”
Rumours of an appearance in the house were fuelled when Ms Markle offered vague responses to questions on the topic during a live interview on Good Morning Britain last month.
Host Kate Garraway grilled the 54-year-old over rumours that she would enter the CBB house amid claims she was “cashing in” on her estranged half-sister’s marriage to Prince Harry.
Despite remaining tight-lipped on the topic Ms Markle hinted at an appearance saying "money makes the world go round".
Ms Markle has in the past been outspoken about Prince Harry and Meghan Markle in numerous scathing social media rants.
Most recently, she branded her half sister “inhumane” for the treatment of their father, Thomas Markle, 74.