Kane suffered ankle ligament damage last month but has made a speedy recovery to hand Mauricio Pochettino's side a boost ahead of the run-in.
Vincent Janssen will lead Tottenham's frontline, having starred in Wednesday's impressive comeback at Swansea.
Captain Hugo Lloris has recovered from illness to take his place between the sticks with Spurs set to line up in a 4-2-3-1 formation.
Spurs, who are unbeaten on home turf this season, are aiming to cut Chelsea's lead to four points ahead of the Blues' trip to Bournemouth this evening.
Watford occupy 10th place in the table and are closing in on the magical 40-point mark but are without several key players at White Hart Lane.
Tottenham XI: Lloris (C), Trippier, Alderweireld, Vertonghen, Davies, Dier, Dembele, Son, Eriksen, Dele, Janssen.
Tottenham subs: Lopez, Walker, Wimmer, Nkoudou, Sissoko, Wanyama, Kane.
Watford XI: Gomes (GK) (c); Mariappa, Cathcart, Holebas; Janmaat, Cleverley, Doucouré, Amrabat; Niang, Okaka, Success.
Watford subs: Pantilimon (GK), Kabasele, Eleftheriou, Mason, Zúñiga, Capoue, Deeney.
What they're saying
Mauricio Pochettino: "In football you cannot stop learning and improving, always every day is a new challenge.
"It’s true that with more experience it’s always better but for us it’s important to be focused on us and try to win the next three points.
"We must be focused on us – only on us and try give the best to win the game.”
Walter Mazzarri: “Against Tottenham, we are always in an emergency.
“We had one of the best games since I’ve been here on Tuesday [against West Brom].
"We played the game the way I like and I would have liked to have been able to play against Tottenham with a full team."