The Italian giants are the clear favourites to progress after winning their group convincingly, while Lyon scraped through a weak group in second place with just eight points.
The French side’s performance in Ligue 1 has been below par this season, as they sit seventh in the table, some 28 points behind league leaders Paris Saint-Germain.
The two teams last met in the group stage of the 2016 Champions League where Juve easily won the group and Lyon were only good enough to qualify for the Europa league.
Here’s everything you need to know about the game.
How can I watch on TV?
The game will be shown live on TV in the UK, and can be watched on BT Sport 3 – coverage starts at 7pm.
Can I stream it online?
Yes, BT Sport subscribers will be able to stream the match online via the video player and BT Sport app.
Lyon will be without one of their star players in Memphis Depay who misses out through a long-term knee injury. Jeff Reine-Adelaide and Youssef Kone are side-lined with knee and ankle injuries respectively, while Rafael and Oumar Solet are doubts for the game.
Douglas Costa (knee) and Gonzalo Higuain (back) are definitely out for the Italians. Pjanic has returned to training but the game appears to have come too soon for the Bosnian.
Lyon: Lopes; Denayer, Marcelo, Marcal; Duboism Tousart, Guimaraes, Comet, Terrier, Toko-Ekambi, Dembele. (3-4-1-2).
Juventus: Szczesny; Danilo, Bonucci, De Ligt, Alex Sandro; Matuidi, Ramsey, Bentancur, Cuadrado; Dybala, Ronaldo. (4-4-2).
What time is Lyon vs Juventus?
The match kicks off at 8pm GMT at the Groupama Stadium.
Lyon 1-2 Juventus. Expect Lyon to nick a goal in the game, but Juve’s quality means that they should come away from France with a victory.