The singer announced on Monday that her two remaining gigs, planned for 10 and 11 March in Paris, France, have been cancelled.
This comes after French authorities barred gatherings of more than 1,000 people as part of their response to the outbreak.
“Following official notification from the Office of the Police this morning prohibiting all events with an audience attendance of over 1000, Live Nation regrets to announce the final two Madame X performances previously re-scheduled to 10 March and 11 March are forced to be cancelled,” reads a message on Madonna’s website.
Those who had bought tickets will be able to obtain a refund at the point of purchase.
“We regret the disappointment to fans,” adds the note.
Madonna previously had to cancel two shows in Paris on 1 and 7 March, citing “ongoing injuries”.
Other artists, including Mariah Carey, BTS and Stormzy, have had to modify their performing schedules due to the outbreak.
In the US, the South by Southwest festival has been cancelled. It was scheduled for 13-22 March in Austin, Texas.