The track, which was released on January 13, has secured its seventh week at the top spot on the official UK singles chart.
Flowers is Cyrus’s lead single from her upcoming eighth studio album, Endless Summer Vacation, and her third UK number one.
The track is now the longest-running number one from a female solo artist since Adele’s Easy on Me, which spent eight non-consecutive weeks at the top of the Official Charts between 2021 and 2022.
The 30-year-old pop sensation, who found widespread fame starring in Disney Channel’s Hannah Montana, previously topped the UK singles chart with We Can’t Stop in 2013 and Wrecking Ball in the same year.
Elsewhere on the official singles chart, Boy’s A Liar by PinkPantheress has retained its number two spot.
Die For You by The Weeknd has entered the top 10 for the first time since its original release in 2016, thanks to renewed popularity online and the issuing of a new remix featuring Ariana Grande.
The track, which sits at number four, is the artist’s – whose real name is Abel Tesfaye – 14th top 10 hit in the UK.
At number seven is Lizzy McAlpine’s first UK top 10 single Ceilings, while just outside the top 10, Libianca’s People has climbed two places to number 11.
The first single, Heaven, from Niall Horan’s upcoming third solo album The Show, has also crept closer to the top 10, currently sitting at number 16.
Over on the official album charts, Gorillaz have scored their second UK number one album with Cracker Island.
The virtual band’s latest LP has become their first chart-topper in 17 years, having last reached number one with Demon Days in 2005, according to the Official Charts Company.
The rock group was formed in 1998 by Blur’s Damon Albarn and artist Jamie Hewlett, and consists of 2-D, Murdoc Niccals, Noodle and Russell Hobbs.
Speaking to the Official Charts Company following the news, Murdoc Niccals said: “Never in doubt, really. Not only was this foretold in the prophecies, we have the greatest fans in the cosmos.
“Plus, an army of Cracker Island crack teams all over the world doing their bit for the cause.
“It really does take a global village to raise a number one smash hit album. Praise be to every single one of you.”
Cracker Island has also topped the official vinyl albums chart.
Los Angeles-born singer-songwriter Gracie Abrams has reached number three with her debut album Good Riddance, while Adam Lambert has achieved a solo-career best with his fifth studio album High Drama, which sits at number five.
Lambert, 41, previously enjoyed solo top 40 success with his 2009 debut For Your Entertainment, his 2012 release Trespassing and 2015 LP The Original High.
Elsewhere, Obey Robots – a duo consisting of Laura Kidd, of Penfriend and She Makes War, and Ned’s Atomic Dustbin’s Gareth ‘Rat’ Pring – have achieved their first top 20 album with One In A Thousand securing a spot at number 14.