The iconic orange Toyota Supra was driven by the movie star — who tragically died in 2013 aged 40 in a car crash - in the 2001 hit film and its sequel 2 Fast 2 Furious.
The car was sold at auction in Las Vegas over the weekend, with the winning bid of $550k (£396k) placed anonymously by phone.
The 1994 vintage sports car was built by Eddie Paul at his Shark Shop in El Segundo, California and comes with the stock 2JZ-GTE engine.
Dubbed 'The 10 second car' it was mostly used for stunts in the Fast and the Furious films but appeared on screen driven by Walker in the seen his character Brian O'Connor destroys a Ferrari in a street-light race down the Pacific Coast Highway in Malibu.
A new Toyota Supra 2.0 is priced at £49,995.
Walker's brother Cody told TMZ ahead of the auction: "I think it's Stunt 1, so it has all the real go fast parts on it, it's the real deal,' adding an earlier version that wasn't a 'picture car' went for $200k.
"This being an actual screen-used Stunt 1 car... I really wouldn't know what to say. It could go for a lot. I think Paul would think it's hilarious, to be honest with you."
Walker died half way through filming the seventh instalment of the F&F franchise. His brothers Cody and Caleb stepped in to help complete the movie.
The franchise also stars Vin Diesel, Jordana Brewster, Michelle Rodriguez, Tyrese Gibson, Ludacris and Nathalie Emmanuel.
The ninth movie will be in cinemas on 24 June, and it has been confirmed the franchise will come to an end with after the 11th instalment.
Watch: Vin Diesel hits at F&F cameo for Paul Walker's daughter