The broadcaster took aim at the Queen of Pop, 64, on his Uncensored show after she announced a huge greatest hits tour, leaving fans to accuse him of being "misogynistic".
Before the show, he tweeted: "I think Madonna’s become the biggest cringe-making fiasco in world entertainment."
Then during the programme he showed a montage of some of Madonna's recent social media videos and photos and could be heart muttering things like "dear oh dear" and "rock bottom" as it played.
I think Madonna’s become the biggest cringe-making fiasco in world entertainment. We’ll debate that tonight. pic.twitter.com/ptGfQrjsvj
— Piers Morgan (@piersmorgan) January 18, 2023
"I find her behaviour, for a woman in her 60s, just utterly embarrassing," he said.
After a discussion with music mogul Louis Walsh about the singer's fitness level, Morgan asked why she couldn't "put on a lot of clothing" and "go and sit in a rocking chair" and do her show that way.
However, the 57-year-old has fallen foul of fans who have claimed he wouldn't say the same to a male performer the same age as Madonna.
"You do realise how ageist and misogynistic that whole segment was right?" tweeted one viewer.
Another posted: "Covering the Stones tomorrow?
"Or will it just be women who are still performing after the age of 34?"
Somebody else tweeted: "She’s the icon. You’re the tragic one, obsessed with her."
Another claimed the TV star "wouldn't say the same were it a male".
"How are you in any way qualified to discuss that?" asked another fan.
"I personally don't listen to Madonna's music but she has every right as a musician to do whatever she wants without needing permission from you."
Yahoo has contacted representatives for Morgan for comment.
Watch: Madonna is heading out on tour to celebrate her four-decade career