It is tradition for This Morning to plug Loose Women near the end of the show and feature a quick exchange between the respective hosts.
Sitting with co-host Josie Gibson and guest Adam Lambert, Willoughby said: "Let's find out what Loose Women are talking about today. 'Hi there, Ruth!'"
Langsford replied: "Yeah, we love Adam Lambert, too!" And went on to give a rundown of their show before handing back to Willoughby.
It marked Willoughby's first time back on the sofa since the scandal regarding her former colleague Phillip Schofield having an affair with a much younger male colleague.
It was also the first time Willoughby has had to work with Langsford since her husband Eamonn Holmes became one of the most vocal critics of Schofield, Willoughby and This Morning.
Just this morning on his GB News show, Holmes called for "anyone watching today who has kissed Phillip Schofield, let us know".
Viewers were quick to pick up on the awkwardness between Willoughby and Langsford.
One viewer wrote on social media: "I don’t know how Ruth Langsford keeps a straight face when Holly Willoughby speaks to her when introducing Loose Women."
Another said: "Wow Holly having to crossover to Ruth on #loosewomen, considering how Ruth's husband has been sniping about Holly, that must of been awkward."
One viewer simply put: "Ruth and Holly. #awkard"
In recent weeks, Holmes has launched several attacks on Willoughby including mocking her while presenting on GB News and blasting her as "false".
It has also been alleged that Langsford complained about Schofield's conduct in the past. The younger colleague who Schofield had a sexual relationship with also later went on to work at Loose Women.
Schofield has said their relationship was "not illegal" and only became sexual when the colleague was "20 or 21".
Watch below: Holly Willoughby 'shaken, troubled, let down and worried' in This Morning return