The actor, 25, has starred as the web-slinger in several films since 2016.
The latest film – No Way Home - sees the return of previous Spider-Man stars Tobey Maguire and Andrew Garfield.
Speaking on This Morning, Holland said: “You finally have the three Spider-Mans sharing the screen.”
“It was a lot of fun for me,” he added. “Playing Spider-Man is an interesting experience.
“It can be quite alienating, you know, there is only three of us that have ever done it."
If you still haven't seen "Spider-Man: No Way Home" then be warned, this clip contains MAJOR spoilers!! 🕷🕷🕷 pic.twitter.com/VTNa8gGQun
— This Morning (@thismorning) February 3, 2022
"So to have that sort of brotherhood between the three of us and to share the screen and share that moment together was very special," he went on.
The actor was also quizzed about whether No Way Home would be his last outing as the character.
“It could be, but then the first one could have been my last,” he replied.
Read more: Spider-Man sequels that never got made
“It has been a real dream come true.
“If it is time to give the next lucky kid an opportunity to do it I will step down proudly.”
Maguire played Spider-Man in a trio of films between 2002 and 2007. Garfield took over in The Amazing Spider-Man, which was released in 2012, and its 2014 sequel.
Watch: Tom Holland says there have been 'conversations' about his future as Spider-Man