SNP leader Nicola Sturgeon warned Boris Johnson on Sunday he could not keep Scotland in the UK against its will.
The First Minister continued to push for a new independence referendum when she appeared on BBC's Andrew Marr Show.
The Prime Minister and his government have repeatedly said they will not approve another vote on Scottish independence, but Sturgeon argued after the SNP won 48 of Scotland's 59 seats in the UK parliament, her party had been given a mandate for one.
Sturgeon, who previously declared she was out to stop Brexit and advance Scottish independence, said: "If he thinks ... saying no is the end of the matter then he is going to find himself completely and utterly wrong.”
"You cannot hold Scotland in the union against its will ... If the United Kingdom is to continue it can only be by consent.
“And if Boris Johnson is confident in the case for the union then he should be confident enough to make that case and allow people to decide."
Below is a clip from her appearance on the Andrew Marr show.
“If Boris Johnson thinks saying no is the end of the matter then he is going to find himself completely and utterly wrong”— BBC Politics (@BBCPolitics) December 15, 2019
SNP leader Nicola Sturgeon tells #Marr “Scotland can not be imprisoned within the UK against its will”#indyref2 https://t.co/CWOOqT3QJU pic.twitter.com/8Zr44bP4kK
The warning followed a conversation Sturgeon had with Johnson on Friday regarding a new independence referendum following his impressive win in the general election.
Of her phone call with the PM, she added: “I wasn’t sure how much he’d had a chance to catch up with the Scottish results.
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“I pointed out to him, politely of course after I congratulated him, that the Scottish Tories, having fought the election on the single issue of opposition to an independence referendum, had lost – lost vote share, lost more than half of their seats.
“It was a watershed election on Thursday and it’s very clear that Scotland wants a different future to the one chosen by much of the rest of the UK.”
Downing Street said that in the call, Johnson “made clear how he remained opposed to a second independence referendum”.