The ad, which appears on the side of a red London bus, says: "Another Whopper on the side of a bus. Must be an election.”
Pictured next to it is the iconic of Burger King burger.
The advert mocks Vote Leave's claims, which appeared on the side of their battle bus during the EU referendum, that the UK sends £350 million a week to the EU.
The claim has been regularly criticised since, with UK Statistics Authority chairman Sir David Nosgrove describing it as a "clear misuse" of official figures.
Read more from Yahoo News UK:
There was also an attempt made for Boris Johnson to face criminal prosecution over the claim, though this was dropped after the Prime Minister won a High Court challenge.
The advert has been praised on social media, with one person saying: "I'm voting Burger King for Prime Minister.”
Others also piled in:
Weapons grade trolling @BurgerKingUK . A nation salutes you.— Adam (@SkyHiFreeFlyGuy) December 4, 2019
Who👏🏿is👏🏿Responsible👏🏿For👏🏿THIS!!! Give this person a @Cannes_Lions right away— Stylechitect (@Stylechitect) December 4, 2019
Brilliant! pic.twitter.com/fbTlgCc7MV— 🖤 Unicorn Stopper 🎪 🏴 🇪🇺 🕷🎶 (@LenoreSimson) December 4, 2019
A Burger King spokesman said: "As the Home of the Whopper, we felt that if anyone has the right to stick whoppers on the side of a bus, it's us.
"So naturally, we've taken the opportunity to shine a light on our iconic Whopper in all its flame-grilled glory."