An England flag outside Wembley Stadium was at half mast on Sunday following the London terror attack.
Flying alongside the statue of World Cup-winning captain Bobby Moore, it is the latest in days of tributes across the capital to the four lives lost on Wednesday in Westminster.
The Football Association promised "robust" security for the World Cup qualifier, with England facing Lithuania at the national stadium, and increased police presence was evident.
London mayor Sadiq Khan is set to lay a wreath in the centre circle ahead of kick-off in a further tribute.
Khan will be joined by FA chairman Greg Clarke, Metropolitan Police Commissioner Craig Mackey, and Secretary of State for Culture, Media and Sport Karen Bradley.
Kahn said ahead of the game: "As football fans, we will stand together at Wembley to remember those who were killed or injured in Wednesday’s horrific attack to send a message around the world - that Londoners will never be cowed by terrorism.
"Ever since the devastating events of Wednesday afternoon, I have been so humbled and moved by the outpourings of solidarity and compassion from Londoners and visitors to our city alike.
"I want to express my deepest gratitude on behalf of all Londoners to the police and emergency services for the way they responded to the attacks in Westminster. They ran to confront danger while encouraging others to run to safety. All Londoners are grateful for their efforts."