Human brain clumps transplanted into rat brains 'respond to light and sound'

An image of a rat brain with a grafted human brain organoid (Cell)
An image of a rat brain with a grafted human brain organoid. (Cell)

Human brain cells implanted into rat brains can respond to visual stimuli such as lights, a study has shown.

The research shows that 'organoids' of human brain cells – clumps of lab-grown neurons – can integrate with rat brains.

Scientists cultivated human stem cell-derived neurons in the lab for around 80 days before grafting them into the brains of adult rats that had sustained injuries to their visual cortex.

Their research holds promise for treatment of injured human adult brains – although whether the organoid grafts can functionally integrate with the visual system of injured adult brains has yet to be explored.

Previous research has shown that we can transplant individual human and rodent neurons into rodent brains, and, more recently, it has been demonstrated that human brain organoids can integrate with developing rodent brains.

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H Isaac Chen, a physician and assistant professor of neurosurgery at the University of Pennsylvania, said: "We focused on not just transplanting individual cells, but actually transplanting tissue.

"Brain organoids have architecture; they have structure that resembles the brain. We were able to look at individual neurons within this structure to gain a deeper understanding of the integration of transplanted organoids."

In the experiment, the grafted organoids had integrated with their host's brain within three months – becoming vascularised, growing in size and number, sending out neuronal projections, and forming synapses with the host's neurons.

The team made use of fluorescent-tagged viruses that hop along synapses, from neuron to neuron, to detect and trace physical connections between the organoid and brain cells of the host rat.

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Chen said: "By injecting one of these viral tracers into the eye of the animal, we were able to trace the neuronal connections downstream from the retina.

"The tracer got all the way to the organoid."

The researchers used electrode probes to measure the activity of individual neurons within the organoid when the animals were exposed to flashing lights and alternating white and black bars.

Chen said: "We saw that a good number of neurons within the organoid responded to specific orientations of light, which gives us evidence that these organoid neurons were able to not just integrate with the visual system, but they were able to adopt very specific functions of the visual cortex."

The team was surprised by the degree to which the organoids were able to integrate within only three months.

Chen said: "We were not expecting to see this degree of functional integration so early.

"There have been other studies looking at transplantation of individual cells that show that even nine or 10 months after you transplant human neurons into a rodent, they're still not completely mature.

"Neural tissues have the potential to rebuild areas of the injured brain. We haven't worked everything out, but this is a very solid first step.

"Now, we want to understand how organoids could be used in other areas of the cortex, not just the visual cortex, and we want to understand the rules that guide how organoid neurons integrate with the brain so that we can better control that process and make it happen faster."

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