The reality star revealed that she is set to have her first child with boyfriend Tristan Thompson in front of her mother and sisters in Sunday’s episode.
Khloe first revealed the news to assistant, Alexa Okyle, holding up the test and saying: "Oh my God, there are two lines?"
Khloe, who was unable to hold back her excitement, said: "Yeah, clear as day. I am pregnant. I don't want anyone to know because I wanna tell them when I'm with Tristan.”
The 33-year-old later confessed to the camera that she is “super nervous” but also “really excited”.
She said: "I am super nervous, but really excited. There's a million different emotions that are going through my brain but I think I'm almost in a state of shock. I almost can't believe it but then it's good shock.”
When Khloe revealed the news to the rest of the Kardashian clan, she simply said: “We are having a baby.”
The announcement quickly left ‘momager’ Kris Jenner in tears while sister Kourtney joked: “No wonder you gained a few pounds!”
Absent sister Kylie Jenner was later told of the news, to which Jenner screamed “stop” before bursting into tears.
"I'm gonna cry. I'm so happy for you,” she said.
The news comes amid speculation that Kylie Jenner is also pregnant, having been away from the spotlight in recent months.