The full page ad, published in The Guardian, criticises Mr Corbyn for a "toxic culture you have allowed to divide our movement", saying it has prompted the resignation of "thousands" of members.
The party, it says, is no longer a "safe place" for its members and supporters, and Mr Corbyn was accused of "failing the test of leadership”.
If you support @JeremyCorbyn in any way you are not antiracist. And we believe that MPs and peers in @UKLabour are now condoning and complicit with Corbyn’s nasty politics of hate.— Rabbi Zvi Solomons 🕎 (@RabbiZvi) July 17, 2019
This advert is clever, but won’t change anything. pic.twitter.com/0HGVdQjTe7
"We are saying you are accountable as Leader for allowing antisemitism to grow in our party and presiding over the most shaming period in Labour's history," it adds.
The advert is supported by a total of 67 Labour members of the House of Lords, including Peter Hain, Peter Mandelson and Robert Winston, and comes after a damning report by BBC's Panorama programme into the party's handling of allegations of anti-Semitism.
The peers also accuse Mr Corbyn of not having "opened (his) eyes" or "accepted responsibility" for the row which has engulfed the party.
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"We can't be a credible alternative government that will bring the country together if we can't get our own house in order," the advert says.
"Your failure to do the right thing will lead to the failure of the Labour Party being able to make our country a better place for the people and communities we seek to serve.”
"You have failed to defend our party's anti-racist values," it adds.
"You have therefore failed the test of leadership.”
Addressing Mr Corbyn directly, the advert states: "The Labour party welcomes everyone* irrespective of race, creed, age, gender identity, or sexual orientation. (*except, it seems, Jews). This is your legacy Mr Corbyn.”
Labour has hit back at the advert, dismissing the “false and misleading claims about the party by those hostile to Jeremy Corbyn’s politics”.
Insisting the party “stands in solidarity with Jewish people”, Labour added in a statement: “Jeremy Corbyn has made it clear in interviews, videos and articles that there is no place for anti-Semitism in the party.”
The statement went on to highlight how General Secretary Jennie Formby has “sped up” the rate at which cases of anti-Semitism are investigated, and that disciplinary procedures “relate to about 0.06% of members”.
The Labour leader has faced mounting pressure since the Panorama programme, which claimed that senior figures, including Mr Corbyn's communications chief Seumas Milne and general secretary Jennie Formby, had interfered in anti-Semitism investigations.
Labour has denied the claims and written a complaint to the BBC.
Lord Harris of Haringey, chairman of the Labour Peers Group, said on Tuesday that Mr Corbyn was "not cut out" to be a party leader, while Tory leadership hopefuls Boris Johnson and Jeremy Hunt have suggested Mr Corbyn may be anti-Semitic - a claim described as a "baseless political attack" by Labour.
Labour peers have already offered to investigate allegations of anti-Semitism in the party and warned Mr Corbyn that without full openness it was "a cancer that will continue to grow”.
Meanwhile, Labour's governing body has been urged to support a motion which would automatically exclude members where there is "irrefutable evidence" accusing them of racism and other forms of discrimination.
Tom Watson, the party's deputy leader, was among five members of the National Executive Committee who submitted the motion to the chair of the NEC.
The proposed change, which will be debated at a meeting next Tuesday, calls on the NEC to bring forward forward rule changes to the annual conference.