At least 53 people have been injured during clashes between Palestinian worshippers and Israeli police at the Al-Aqsa mosque compound.
The violence at the major holy site, sacred to Muslims and Jews, is an escalation of weeks of violence in Jerusalem that has reverberated across the region.
The Palestinian Red Crescent emergency service said 53 people were wounded at the compound and elsewhere in Jerusalem.
It said most were wounded after being hit in the face and eyes by rubber-coated bullets and shrapnel from stun grenades.
Israel said six police officers were wounded.
Earlier on Friday, Israeli troops shot and killed two Palestinians and wounded a third after the men opened fire on a base belonging to Israel's paramilitary Border Police force in the occupied West Bank.
It was the latest in a series of deadly confrontations in recent weeks that has coincided with the Muslim holy month of Ramadan.
Tensions have soared in recent weeks in east Jerusalem, which is claimed by both Israel and the Palestinians.
At the beginning of the Muslim holy month of Ramadan, Israel blocked off a popular gathering spot where Palestinians traditionally socialise at the end of their day-long fast.
The move set off two weeks of clashes before Israel lifted the restrictions.
But in recent days, clashes have resumed after Israel threatened to evict dozens of Palestinians in the Sheikh Jarrah neighbourhood in east Jerusalem.
The US said it was "deeply concerned" about the heightened tensions and called on all sides to work to de-escalate them.
It also expressed concern about the threatened evictions.