Mr Trump retweeted several posts critical of Dr Anthony Fauci, the White House coronavirus task force's lead adviser, and other health experts within his administration on Monday, appearing to confirm reports of a divide between the US president and his top advisers on the pandemic.
Mr Trump shared unfounded accusations by a former game show host that "everyone is lying," including the country's doctors and the US Centers for Disease Control and Prevention (CDC), the lead agency in the pandemic response.
"The most outrageous lies are the ones about Covid 19. Everyone is lying. The CDC, Media, Democrats, our Doctors, not all but most, that we are told to trust," Chuck Woolery wrote in his tweet.
Mr Trump also retweeted a post by a podcast host which claimed that Dr Fauci and the Democrats require the public to use an ID card "to go shopping but not to vote".
The most outrageous lies are the ones about Covid 19. Everyone is lying. The CDC, Media, Democrats, our Doctors, not all but most ,that we are told to trust. I think it's all about the election and keeping the economy from coming back, which is about the election. I'm sick of it.— Chuck Woolery (@chuckwoolery) July 13, 2020
It comes after several of Mr Trump's aides began briefing the press against Dr Fauci, who has repeatedly diverged from the president over the US response to the coronavirus pandemic.
Over the weekend, White House staff released a lengthy list of remarks that Dr Fauci had made about the virus in the early days of the outbreak to US media outlets in what appeared to be a coordinated attempt to discredit the adviser.
It followed a recent interview by Dr Fauci in which he revealed he had not spoken to Mr Trump in several weeks and said the US response to the virus compared poorly with other countries.
Kayleigh McEnany, the White House press secretary, denied that opposition research against Dr Fauci was "being dumped to reporters" during a briefing on Monday. However she reiterated Mr Trump's previous comment that "Dr Fauci has made some mistakes."
Asked about Mr Trump's retweet of a post claiming the CDC was lying, Ms McEnany said the president was expressing his displeasure with "some rogue individuals" within the CDC who had misled the American public.
"The notion of the tweet was to point out the fact that when we use science we have to do it in a way that is not political," she said.
Mr Trump later told the reporters at the White House: "I have a very good relationship with Dr Fauci, I always have right from the beginning. I don't always agree with him... but I get along with him very well. I like him personally."
The reported rift between the Trump administration and Dr Fauci comes against a backdrop of rising coronavirus cases in around 39 US states.
The worst hit state is Florida, which reported more than 15,000 new cases of Covid-19 on Sunday, the highest daily number of any state since the pandemic began.
The previous one-day record was set by New York at the height of its outbreak in April, when it reported 12,274 cases in one day.
The number of Covid-19 related hospitalisations in Florida is also a cause for concern, with 47 hospitals in the state reporting their intensive care units were completely full, including eight hospitals in the Miami-Dade County hot spot.