The central London march, which will start in Park Lane and end in Parliament Square, is expected to be the biggest public protest so far against the current Brexit situation.
Activists will march through the capital demanding the government give the British people another vote on the final deal with the European Union. This will likely take the form of another referendum.
The Evening Standard revealed earlier this month that a majority of Brits now want the country to stay in the EU, following analysis of nearly 150 polls.
This week actor Steve Coogan was among those encouraging people to attend Saturday’s march, which will be led by chef Delia Smith and Dragon’s Den star Deborah Meaden.
Mayor Sadiq Khan has also given his support, while other supporters include Labour MP Chuka Umunna, Tory MPs Sarah Wollaston and Anna Soubry, Lib Dem leader Sir Vince Cable and Green Party MP Caroline Lucas.
Twice as many coaches have been booked for the event than were booked for the march against Brexit in June, which saw 100,000 people attend.
Sir Patrick Stewart, Armando Iannucci and Jamie Carragher have been among those helping to sponsor coaches.
The People’s Vote campaign, which is organising the march, has been backed by celebrities include Lena Headey, Bob Geldof, Michael Morpurgo, Tim Minchin and Dominc West.
Protesters will begin assembling from midday just north of the Hilton Hotel on Park Lane.
The route see them march down Park Lane along the eastern edge of Hyde Park. They will then turn left and walk along Piccadilly and onto Trafalgar Square via St James Street and Pall Mall.
The march will proceed down Whitehall, past Downing Street and end in Parliament Square. There speakers will deliver addresses in support of a people’s vote from 2pm onwards.
People’s Vote UK is a grassroots campaign with support from across the political spectrum. It has more than a million social media followers and is growing every day.
Many believe the UK is heading for a no deal or bad deal Brexit - which is not what the country voted for in June 2016.
They want the people to be able to vote on the government's final Brexit deal before.